Neurochemistry in Ciudad Real

Research interest

The Neurochemistry Group of Ciudad Real (GNCR) is focused on the study of signal transduction pathways mediated by adenosine and metabotropic glutamate receptors in central nervous system and heart. Receptor/effector system characterization and pathways regulation are the main topics in our research. Primary cortical neuron culture, C6 glioma cells, granular cerebellar cells or cardiomyocytes are used as in vitro systems, and rat brain and heart are used as in vivo systems.

The first research line analyzes the modulation of adenosine and glutamate signalling pathways that take place during pregnancy and lactation. Rats are treated during pregnancy and lactation and the effect of different drugs with agonist/antagonist activity on these receptors is analyzed. This study is performed in brain and heart tissues from mothers, fetuses and neonates treated with caffeine, theophylline or glutamate.

The second research line deals with the implication of adenosine receptors and metabotropic glutamate receptors in excitotoxicity and cell death mechanisms. To this end we use neuron, C6 cells and cardiomyocytes cultures. These cells are subjected to pharmacological treatment or hypoxic insult.

Finally, our group analyzes the role of these receptors in neurodegenerative diseases, as Alzheimer, Parkinson, Pick diseases and other dementias. To this end, we analyze postmortem brains from human patients to assess the status of these receptors and their effector systems. The aim is to develop a possible differential diagnostic tools and new therapeutical strategies against these dementias.

 

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Funding

  • Título: Regulación de receptores de adenosina y metabotrópicos de glutamato durante el envejecimiento: implicación en procesos de degeneración y muerte celular
    Entidad financiadora: Consejería de Educación, Cultura y Deportes
    I.P.: Mairena Martín López
    Duración, desde: 27/09/2014   hasta: 26/09/2016
  • Título: Papel neuroprotector de la cafeína como modulador de receptores de adenosina en modelos in vivo
    Entidad financiadora: Diputación de Ciudad Real
    I.P.: Inmaculada Ballesteros Yañez
    Duración, desde: 04/07/2014   hasta: 31/01/2015
  • Título: Control de la neuromodulación de las vías mediadas por los receptores de adenosina y metabotrópicos de glutamato: potencial terapéutico en enfermedades neurodegenerativas
    Entidad financiadora: Ministerio de Ciencia e Innovación
    I.P.: Mairena Martín López
    Duración, desde: 01/01/2012   hasta: 31/12/2014
  • Título: Epigenetic analysis and functionality of dopamine D2, adenosine A1 and A2A receptors in schizophrenia: molecular mechanims involved
    Entidad financiadora: Institut d’Investigació Biomèdica de Bellvitge
    I.P.: Mairena Martín López
    Duración, desde: 01/01/2010   hasta: 31/12/2012

 

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Publications

  • León-Navarro DA, Albasanz JL, Martín M.. Hyperthermia-induced seizures alter adenosine A1 and A2A Receptors and 5′-nucleotidase activity in rat cerebral cortex. J Neurochem. 2015 Apr 23. doi: 10.1111/jnc.13130. [Epub ahead of print]. (A).
  • Iglesias I, Albasanz JL, Martín M. Effect of Caffeine Chronically Consumed During Pregnancy on Adenosine A1 and A2A Receptors Signaling in Both Maternal and Fetal Heart from Wistar Rats. J Caffeine Res. 2014; 4(4):115-126. (A).
  • Villar-Menéndez I, Nuñez F, Díaz-Sánchez S, Albasanz JL, Taura J, Fernández-Dueñas V, Ferrer I, Martín M, Ciruela F, Barrachina M. Striatal adenosine A2A receptor expression is controlled by S-adenosyl-L-methionine-mediated methylation. Purinergic Signal. 2014 Sep;10(3):523-8. doi: 10.1007/s11302-014-9417-4. (A).
  • Villar-Menéndez I, Porta S, Buira SP, Pereira-Veiga T, Díaz-Sánchez S, Albasanz JL, Ferrer I, Martín M, Barrachina M. Increased striatal Adenosine A2A receptor levels is an early event in Parkinson’s disease-related pathology and it is Potentially Regulated by miR-34b. Neurobiol Dis. 2014;69:206-14. doi: 10.1016/j.nbd.2014.05.030. (A).
  • Villar-Menéndez I, Díaz-Sánchez S, Blanch M, Albasanz JL, Pereira-Veiga T, Monje A, Planchat LM, Ferrer I, Martín M, Barrachina M. Reduced striatal adenosine A2A receptor levels define a molecular subgroup in schizophrenia.J Psychiatr Res. 2014;51:49-59. doi: 10.1016/j.jpsychires.2013.12.013. (A).
  • Villar-Menéndez I, Blanch M, Tyebji S, Pereira-Veiga T, Albasanz JL, Martín M, Ferrer I, Pérez-Navarro E, Barrachina M. Increased 5-methylcytosine and decreased 5-hydroxymethylcytosine levels are associated with reduced striatal A2AR levels in Huntington’s disease. Neuromolecular Med. 2013;15(2):295-309. (A).
  • Ballesteros-Yáñez I, Castillo CA, Amo-Salas M, Albasanz, JL, Martín M. Differential effect of caffeine consumption on diverse brain areas of pregnant rats. J Caffeine Res. 2012; 2(2): 90-98. (A).
  • Barrachina M, Martín M, Ciruela F, Ferrer I. Epigenetic Modulation of Adenosine A2A Receptor: A Putative Therapeutical Tool for the Treatment of Parkinson’s Disease. En: Towards New Therapies for Parkinson’s Disease (Finkelstein DI Ed.). 2011. ISBN: 978-953-307-463-4. Ed. Intech Open Access Publishers (Rijeka, Croacia). (CL).
  • Albasanz JL, Castillo CA, Barrachina M, Ferrer I, Martín M. Modulation of Signal Transduction Pathways in Senescence-Accelerated Mice P8 Strain: A Useful Tool for Alzheimer’s Disease Research. En: The Clinical Spectrum of Alzheimer’s Disease -The Charge Toward Comprehensive Diagnostic and Therapeutic Strategies (de la Monte S, Ed). 2011. Págs: 347-362. ISBN: 978-953-307-993-6. Ed. Intech Open Access Publishers (Rijeka, Croacia). (CL).
  • Giust D, Albasanz JL, Martín M, Marega R, Delforge A, Bonifazi D. [60]Fullerene-based monolayers as neuroprotective biocompatible hybrid materials. Chem Commun. 2011. 47[38]: 10617-10619. (A).
  • Ruiz MA, León DA, Albasanz JL, Martín M. Desensitization of adenosine A(1) receptors in rat immature cortical neurons. Eur J Pharmacol. 2011. 670[2-3]: 365-371. (A).
  • López-Zapata A, León D, Castillo CA, Albasanz JL, Martín M. Maternal glutamate intake during gestation and lactation regulates adenosine A(1) and A(2A) receptors in rat brain from mothers and neonates. Neuroscience. 2011. 199: 133-142. (A).
  • Ballesteros-Yáñez I, Benavides-Piccione R, Bourgeois JP, Changeux JP, DeFelipe J. Alterations of cortical pyramidal neurons in mice lacking high-affinity nicotinic receptors. Proc Natl Acad Sci U S A. 2010. 107[25]: 11567-11572. (A).
  • Buira SP, Dentesano G, Albasanz JL, Moreno J, Martín M, Ferrer I, Barrachina M. DNA methylation and Yin Yang-1 repress adenosine A receptor levels in human brain. J Neurochem. 2010. 115[1]: 283-295. (A).
  • Castillo CA, León DA, Ballesteros-Yáñez I, Iglesias I, Martín M, Albasanz JL. Glutamate Differently Modulates Metabotropic Glutamate Receptors in Neuronal and Glial Cells. Neurochem Res. 2010. 35[7]: 1050-1063. (A).
  • Castillo CA, León DA, Ballesteros-Yáñez I, Albasanz JL, Martín M. Glutamate differently modulates excitatory and inhibitory adenosine receptors in neuronal and glial cells. Neurochem Int. 2010. 57[1]: 33-42. (A).
  • Lorenzo AM, León D, Castillo CA, Ruiz MA, Albasanz JL, Martín M. Maternal caffeine intake during gestation and lactation down-regulates adenosine A(1) receptor in rat brain from mothers and neonates. J Neurosci Res. 2010. 88[6]: 1252-1261. (A).
  • Buira SP, Albasanz JL, Dentesano G, Moreno J, Martín M, Ferrer I, Barrachina M. DNA methylation regulates adenosine A(2A) receptor cell surface expression levels. J Neurochem. 2010. 112[5]: 1273-1285. [Epub 2009]. (A).
  • Sánchez-Nogueiro J, Marín-García P, León D, León-Otegui M, Salas E, Gómez-Villafuertes R, Gualix J, Miras-Portugal MT. Axodendritic fibres of mouse cerebellar granule neurons exhibit a diversity of functional P2X receptors. Neurochem Int. 2009. 55[7]: 671-682. (A).
  • León DA, Castillo CA, Albasanz JL, Martín M. Reduced Expression And Desensitization Of Adenosine A1 Receptor/Adenylyl Cyclase Pathway After Chronic R-Pia Intake During Pregnancy. Neuroscience. 2009. 163[2]: 524-532. (A).
  • Castillo CA, Albasanz JL, León D, Jordán J, Pallàs M, Camins A, Martín M. Age-related expression of adenosine receptors in brain from the senescence-accelerated mouse. Exp Gerontol. 2009. 44[6-7]: 453-461. (A).
  • Ballesteros-Yanez I, Ambrosio E, Pérez J, Torres I, Miguéns M, García-Lecumberri C, DeFelipe J. Morphine self-administration effects on the structure of cortical pyramidal cells in addiction-resistant rats. Brain Res. 2008. 1230: 61-72. (A).
  • Albasanz JL, Perez S, Barrachina M, Ferrer I, Martín M. Up-regulation of adenosine receptors in the frontal cortex in Alzheimer’s disease. Brain Pathol. 2008. 18[2]: 211-219. (A).
  • León D, Albasanz JL, Castillo CA, Martín M. Effect of glutamate intake during gestation on adenosine A(1) receptor/adenylyl cyclase pathway in both maternal and fetal rat brain. J Neurochem. 2008. 104[2]: 435-445. [Epub 2007]. (A).
  • Castillo CA, León D, Ruiz MA, Albasanz JL, Martín M. Modulation of adenosine A(1) and A(2A) receptors in C6 glioma cells during hypoxia: involvement of endogenous adenosine. J Neurochem. 2008. 105[6]: 2315-2329. (A).
  • León DA, Albasanz JL, Castillo CA, Iglesias I, Martín M. Effect of chronic gestational treatment with the adenosine A1 receptor agonist R-phenylisopropyladenosine on metabotropic glutamate receptors/phospholipase C pathway in maternal and fetal brain. J Neurosci Res. 2008. 86[15]: 3292-3305. (A).
  • Albasanz JL, Rodríguez A, Ferrer I, Martín M. Up-regulation of adenosine A(1) receptors in frontal cortex from Pick’s disease cases. Eur J Neurosci. 2007. 26[12]: 3501-3508. (A).
  • Perez-Buira S, Barrachina M, Rodriguez A, Albasanz JL, Martin M, Ferrer I. Expression levels of adenosine receptors in hippocampus and frontal cortex in argyrophilic grain disease. Neurosci Lett. 2007. 423[3]: 194-199. (A).
  • Castillo CA, Albasanz JL, Fernandez M, Martin M. Endogenous Expression of Adenosine A(1), A (2 )and A (3) Receptors in Rat C6 Glioma Cells. Neurochem Res. 2007. 32[6]: 1056-1070. (A).
  • Iglesias I, Castillo CA, Leon D, Ruiz MA, Albasanz JL, Martin M. Metabotropic glutamate receptor/phospholipase C system in female rat heart. Brain Res. 2007. 1153: 1-11. (A).
  • León D, Marín-García P, Sánchez-Nogueiro J, de la O FO, García-Carmona F, Miras-Portugal MT. P2X agonist BzATP interferes with amplex-red-coupled fluorescence assays. Anal Biochem. 2007. 367[1]: 140-142. (A).
  • Leon D, Castillo CA, Ruiz MA, Albasanz JL, Martin M.. Metabotropic glutamate receptor/phospholipase C pathway is increased in rat brain at the end of pregnancy. Neurochem Int. 2007. 50[5]: 681-688. (A).
  • Rodriguez A, Martin M, Albasanz JL, Barrachina M, Espinosa JC, Torres JM, Ferrer I. Group I mGluR signaling in BSE-infected bovine-PrP transgenic mice. Neurosci Lett. 2006. 410[2]: 115-120. (A).
  • Albasanz JL, Rodriguez A, Ferrer I, Martin M. Adenosine A2A receptors are up-regulated in Pick’s disease frontal cortex. Brain Pathol. 2006. 16[4]: 249-255. (A).
  • Rodríguez A, Martin M, Albasanz JL, Barrachina M, Espinosa JC, Torres JM, Ferrer I. Adenosine A1 Receptor Protein Levels and Activity Is Increased in the Cerebral Cortex in Creutzfeldt-Jakob Disease and in Bovine Spongiform Encephalopathy-Infected Bovine-PrP Mice. J Neuropathol Exp Neurol. 2006. 65: 964-975. (A).
  • Iglesias I, Leon D, Ruiz MA, Albasanz JL, Martin M. Chronic intake of caffeine during gestation down regulates metabotropic glutamate receptors in maternal and fetal rat heart. Amino Acids. 2006. 30[3]: 257-266. (A).
  • Dalfo E, Albasanz JL, Rodriguez A, Martin M, Ferrer I. Abnormal group I metabotropic glutamate receptor expression and signaling in the frontal cortex in Pick disease. J Neuropathol Exp Neurol. 2005. 64[7]: 638-647. (A).
  • Rodriguez A, Freixes M, Dalfo E, Martin M, Puig B, Ferrer I. Metabotropic glutamate receptor/phospholipase C pathway: A vulnerable target to Creutzfeldt-Jakob disease in the cerebral cortex. Neuroscience. 2005. 131[4]: 825-832. (A).
  • Albasanz JL, Dalfo E, Ferrer I, Martin M. Impaired metabotropic glutamate receptor/phospholipase C signaling pathway in the cerebral cortex in Alzheimer’s disease and dementia with Lewy bodies correlates with stage of Alzheimer’s-disease-related changes. Neurobiol Dis. 2005. 20[3]: 685-693. (A).
  • Luján R, Albasanz JL, Shigemoto R, Juiz JM. Preferential localization of the hyperpolarization-activated cyclic nucleotide-gated cation channel subunit HCN1 in basket cell terminals of the rat cerebellum. Eur J Neurosci. 2005. 21[8]: 2073-2082. (A).
  • León D, Albasanz JL, Ruiz MA, Iglesias I, Martin M. Effect of chronic gestational treatment with caffeine or theophylline on group I metabotropic glutamate receptors in maternal and fetal brain. J Neurochem. 2005. 94[2]: 440-451. (A).
  • Ruiz MA, Albasanz JL, Leon D, Ros M, Andres A, Martin M. Different modulation of inhibitory and stimulatory pathways mediated by adenosine after chronic in vivo agonist exposure. Brain Res. 2005. 1031[2]: 211-221. (A).
  • Leon D, Albasanz JL, Ruiz MA, Martin M. Chronic caffeine or theophylline intake during pregnancy inhibits A1 receptor function in the rat brain. Neuroscience. 2005. 131[2]: 481-489. (A).
  • Leon ND, Albasanz JL, Iglesias I, Ruiz MA, Martin M. Effect of chronic glutamate administration to pregnant rats during gestation on metabotropic glutamate receptors from mothers and full-term fetuses brain. Amino Acids. 2005. 28[2]: 127-137. (A).
  • Dalfo E, Albasanz JL, Martin M, Ferrer I. Abnormal metabotropic glutamate receptor expression and signaling in the cerebral cortex in diffuse Lewy body disease is associated with irregular alpha-synuclein/phospholipase C (PLCbeta1) interactions. Brain Pathol. 2004. 14[4]: 388-398. (A).
  • Leon D, Albasanz JL, Fernandez M, Ruiz MA, Martin M. Down-regulation of rat brain adenosine A1 receptors at the end of pregnancy. J Neurochem. 2004. 88[4]: 993-1002. (A).
  • Albasanz JL, Fernandez M, Martin M. Internalization of metabotropic glutamate receptor in C6 cells through clathrin-coated vesicles. Brain Res Mol Brain Res. 2002. 99[1]: 54-66. (A).
  • Leon D, Albasanz JL, Ruiz MA, Fernandez M, Martin M. Adenosine A1 receptor down-regulation in mothers and fetal brain after caffeine and theophylline treatments to pregnant rats. J Neurochem. 2002. 82[3]: 625-634. (A).
  • Albasanz JL, Leon D, Ruiz MA, Fernandez M, Martin M. Adenosine A1 receptor agonist treatment up-regulates rat brain metabotropic glutamate receptors. Biochim Biophys Acta. 2002. 1593[1]: 69-75. (A).
  • Ruiz MA, Escriche M, Lluis C, Franco R, Martin M, Andres A, Ros M. Adenosine A(1) receptor in cultured neurons from rat cerebral cortex: colocalization with adenosine deaminase. J Neurochem. 2000. 75[2]: 656-664. (A).

Mairena Martín López

Directora

People

Permanent staff

Phone: (+34) 926 295 300

Contact information

Facultad de Ciencias y Tecnologías Químicas de Ciudad Real

Universidad de Castilla-La Mancha

Avenida de Camilo José Cela, 10

13071 Ciudad Real (España)

telefono (+34) 926 295 300, Ext. 6603

fax (+34) 902 204 130

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